Allen, Richard (2021). CIL:39718, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WM1CRH

The bottom of the buccal cavity is surrounded by specialized cytoplasm and contains a lip that constricts the opening leading through the cytostome into the receiving vacuole or nascent vacuole. The oral ribs extend along one side of the cytostome and merge into the cytopharynx. Oral ribs have alveoli between the ribs but alveoli stop at the beginning of the cytopharynx. Lamellae, of 2 microtubules each, border the cytopharynx and direct discoidal vesicles to the site of fusion with the membrane of the growing vacuole. Two electron opaque vesicles, called lysosomes by most authors (eg. Nilsson, Biochem. and Phys. of Protozoa, 2nd ed., Vol 2:339-379, 1979, Acad. Press.), which are probably acidosomes, bind to the nascent vacuole membrane. TEM taken on 8/23/67 by R. Allen with Philips 200 operating at 60kV. Neg. 12,400X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film
Data qualification: Raw;spatialmeasurements

• Cell Image Library Group ID: 10648

• Tetrahymena pyriformis

• Acidosome
• Cell by organism
• Ciliated Protist
• Cytopharynx
• Eukaryotic cell
• Eukaryotic Protist
• Microtubule associated complex
• Oral apparatus
• Primary lysosome

• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39718

Doi: https://doi.org/10.6075/J0WM1CRH

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39718

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2025-01-30