Rusan, Nasser; Wadsworth, Patricia (2021). CIL:40269, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GQ6X48

Time lapse movie of a porcine kidney LLCPK1 cell stably expressing EGFP-alpha tubulin. The movie shows microtubule behavior from early prophase through metaphase.

• 2021

• Rusan, Nasser
• Wadsworth, Patricia

Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a MicroMax cooled CCD camera.
Images were captured every 2 sec and are displayed at 7.5 frames/sec.
See also:N Rusan and P Wadsworth 2005. Centrosome fragments and microtubules are transported asymmetrically away from division plane in mitosis.J Cell Biol 168:21-28.

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed

• Cell Image Library Group ID: 10946

• Sus scrofa domestica

• Kidney epithelial cell
• Microtubule cytoskeleton

• LLC-PK1
• Microtubule cytoskeleton organization
• Mitosis
• Mitotic metaphase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 40269

Doi: https://doi.org/10.6075/J0GQ6X48

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40269

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2025-01-29