Sun, Yuting; Ge, Yejing; Dmevich, Jenny; Zhao, Yong; Band, Mark; Chen, Jie (2021). CIL:13596, Mus musculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VM4B1H

Mouse hind limb tibialis anterior (TA) muscles were injected with BaCl2 followed by daily systemic administration of rapamycin (Rap) starting 7 days after injury. The animals were sacrificed at different days, and the injured muscles were dissected and cryosectioned followed by hematoxylin (blue) and eosin (pink to red) staining.

This image shows the regeneration of muscle tissue seven days after the injury, but before the systematic administration of Rap. The regenerated muscle fibers, stained by eosin and showed as red patches, were formed but not fully matured. Nuclei were stained by hematoxylin in blue. This image corresponds to Fig 10B from JCB 189: 1157-1169, 2010. See also CIL: 13595, 13597, 13598, 13599, and 13600.

• 2021

• Band, Mark
• Chen, Jie
• Dmevich, Jenny
• Ge, Yejing
• Sun, Yuting
• Zhao, Yong

TA muscles were isolated by dissection, frozen in liquid nitrogen–cooled 2-methylbutane, and embedded in TBS tissue freezing medium. Sections of 10-μm thickness were made with a cryostat at -20°C, placed on uncoated slides, and stained with hematoxylin and eosin. The stained slides were examined with a microscope (DMI 4000B), and the images were captured with a Fluotar 20× 0.4 NA dry objective (Leica) using a camera (RETIGA Exi). The images were processed as 24-bit colored images using Photoshop.

Preparation: cryostat-sectioned tissue; Fix in liquid nitrogen–cooled 2-methylbutane
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: bright-field microscopy
Parameter imaged: absorption of illumination
Source of contrast: differences in adsorption or binding of stain; compartmentalization of stain or label
Visualization methods: hematoxylin; eosin
Processing history: Brightness and Contrast Adjusted
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 8415

• Mus musculus

• Nucleus
• Skeletal muscle cell

• Myoblast fusion involved in skeletal muscle regeneration

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13596

Doi: https://doi.org/10.6075/J0VM4B1H

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13596

Reference

• JCB 189: 1157-1169, 2010 https://www.ncbi.nlm.nih.gov/pubmed/?term=20566686

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12