CIL:43851, Mus musculus, mammary epithelial cell
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- Collection
- Cite This Work
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Chakrabarti, Rumela; Sinha, Satrajit; Kang, Yibin (2021). CIL:43851, Mus musculus, mammary epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03N22ST
- Description
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The image shows a section of a developing wildtype mouse mammary gland immunostained for vimentin (red), beta-catenin (green) and DNA (blue). This is an example of a control image for experiments demonstrating the functions of the Elf5 protein. See: Chakrabarti et al. 2012. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14:1212-1222.
- Date Issued
- 2021
- Researchers
- Methods
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Mammary gland tissue from wildtype P17.5 mice was fixed in formaldehyde, embedded in wax, and sections immunostained for vimentin (red), beta-catenin (green), and DNA (DAPI, blue). Images were recorded using a Leica confocal microscope.
See also: Chakrabarti et al. 2012. Elf5 inhibits the epithelial-mesenchymal transition in mammary gland development and breast cancer metastasis by transcriptionally repressing Snail2. Nat Cell Biol 14:1212-1222. - Technical Details
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Preparation: formaldehyde fixed tissue; tissue in wax embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI) - Scientific Name
- Anatomy
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 43851
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL43851
- NIGMS Biomedical Beat: https://biobeat.nigms.nih.gov
Source data
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12