Perlstein, Ethan; Korostyshevsky, Daniel (2021). CIL:40440, Saccharomyces cerevisiae, chc1 mutant. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0B27T52

Transmission electron micrograph of a vacuole from a sertraline-treated chc1 mutant cell. This vacuole shows evidence of active autophagy. This image is a high-magnification zoom of the cell in CIL 40415 (16,000X).This image is part of a group of yeast vacuole images (CIL:40437-40448). The image contributor encourages Library users to add comments regarding possible causes the separation between the homogenous electron-opaque contents and the mixed opacity contents.

• 2021

• Korostyshevsky, Daniel
• Perlstein, Ethan

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 11331

• Saccharomyces cerevisiae

• Autophagic vacuole
• CHC1 mutant

• Organelle organization
• Sertraline treatment

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40440

Doi: https://doi.org/10.6075/J0B27T52

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40440

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

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2022-08-12