Szulwach, Keith E.; Li, Xuekun; Smrt, Richard D.; Li, Yujing; Luo, Yuping; Lin, Li; Santistevan, Nicholas J.; Li, Wendi; Zhao, Xinyu; Jin, Peng (2021). CIL:13523, Mus musculus, neuronal stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JQ0ZVF

A confocal Z-stack of neurons in postnatal hippocampus, expressing a GFP-tagged knockdown construct for the microRNA miR-137 (green, sh-miR-137) and immunostained to localize doublecortin (red), a marker of neuronal differentiation, and labeled with Dapi (blue), to determine the function of this microRNA in vivo. miR-137 regulates a core transcription factor in stem cell renewal, and data from these experiments suggest that high levels of miR-137promotes neuronal stem cell proliferation, but repressed neuronal differentiation. This image is original data from Fig. 5 of Szulwach et al.(2010) Cross talk between microRNA and epigenetic regulation in adult neurogenesis, J. Cell Biol. Vol. 189:127–141.

• 2021

• Jin, Peng
• Li, Wendi
• Li, Xuekun
• Li, Yujing
• Lin, Li
• Luo, Yuping
• Santistevan, Nicholas J.
• Smrt, Richard D.
• Szulwach, Keith E.
• Zhao, Xinyu

A retroviral vector containing sh-miR-137 under a U6 promoter and EGFP under a chicken actin promoter was prepared, and the virus containing the construct was injected into the dentate gyrus of adult mice in vivo. After 1 week, animals were anesthetized, perfused with 4% paraformaldehyde, brains removed and postfixed, equilibrated in 30% sucrose, and sectioned at a thickness of 40µm with a sliding microtome. Infected cells were localized using chicken-α-GFP (Invitrogen) and rabbit-α-DCX (Cell Signaling Technology), secondaries were α-chicken Alexa Fluor 488 and goat-α-rabbit Alexa Fluor 568 (both from Invitrogen). Confocal z-stacks were acquired at 1 µm steps using a Nikon TE2000 with equipped with a spinning disc confocal with an oil immersion 40X 1.3 NA objective (Zeiss). For additional details see: J. Cell Biol. Vol. 189:127–141.

Preparation: formaldehyde fixed tissue; permeabilized tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain; distribution of epitope; distribution of a specific protein
Visualization methods: Alexa Fluor 488; 4',6-diamidino-2-phenylindole (DAPI); Alexa Fluor 568
Data qualification: Raw

• Cell Image Library Group ID: 8575

• Mus musculus

• Neuronal stem cell
• Nucleus

• Regulation of neurogenesis
• Regulation of neuron differentiation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13523

Doi: https://doi.org/10.6075/J0JQ0ZVF

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13523

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=0368621

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

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2022-08-12