Microscopy product ID: 3561
2D Image
Description | Electron micrograph of a selectively-stained spiny dendrite from a medium spiny neuron contained in a 4 um thick section from a dopamine transporter knock out mouse, imaged using ultra high voltage electron microscopy. |
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Description | Tar file containing IMOD files (datkoc2g26.com/.log/.st/.preali/.fid/.rawtlt) used for the alignment and the original tiff images (in the TIFF folder in the format datkoc2g26000.tif.gz) |
Animation
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Description | Animation of aligned electron microscopic tilt series of a selectively-stained spiny dendrite from a medium spiny neuron contained in a 4 um thick section from a dopamine transporter knock out mouse, imaged using ultra high voltage electron microscopy. Tilt series was obtained at 2 degree increments through +/- 70 degrees of tilt. |
Segmentation
Description | Manual tracing of dendritic spines from tomographic volume using IMOD version 3.7, followed by surfacing using IMOD with smoothsurf |
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Description | a .tar file containing the segmentation files of a dendritic shaft and individual spines in IMOD's .mod format (datkoc2g26_full.mod) |
Reconstruction
Description | Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite from a medium spiny neuron contained in a 4 um thick section from the neostriatum of a dopamine transporter knock out mouse. |
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Description | Tar file containing the IMOD .rec format (datkoc2g26_full.rec) for the volume reconstruction and Amira .am/.hx format image stack of the labels used (datkoc2g26_full-labels.am, datkoc2g26.hx). |
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Description | Animation through the computed slices of a tomographic reconstruction of a selectively-stained spiny dendrite from a medium spiny neuron contained in a 4 um thick section from the neostriatum of a dopamine transporter knock out mouse. |
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- Collection
- Cite This Work
-
Price, Diana; Laakso, Aki; Cyr, Michele; Martone, Maryann; Yamada, Naoko; Thor, Andrea; Berlanga, Monica L. (2017). Microscopy product ID: 3561. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0NC610T
- Creation Date
- Microscopy product: 2006-04-10. Experiment: 2003-02-20. Project: 2003-01-01.
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: UHVEM. Instrument: Hitachi UHVEM
Strain: B6;129-/Slc6a3 tm2Mca
- Funding
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NIH
- Series
- Scientific Name
- Anatomy
- Topic
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View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resource
- Microscopy product 3561 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3561
Other version
- License
-
Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
-
Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22