Doyle, Andrew D.; Wang, Francis W.; Matsumoto, Kazue; Yamada, Kenneth M. (2021). CIL:13160, Homo sapiens, keratinocyte, epidermal cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RN36K2

Topographical regulation of keratinocyte migration. 2D, 3D, and 1D fibrillar migration of human epidermal keratinocytes. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 3D surfaces were constructed according to Science. 294: 1708–1712 (2001). 1D surfaces were made by using a 2 photon confocal microscope to pattern a polyvinyl alchohol (PVA) coated coverslip. Movie is video 4 from JCB 2009, 184:481-490

• 2021

• Doyle, Andrew D.
• Matsumoto, Kazue
• Wang, Francis W.
• Yamada, Kenneth M.

Following gluteraldehyde quenching, ECM proteins were absorbed onto the PVA surface. Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 4 min and shown at 15 frames per second.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: custom-made smoothing, sharpening, and convolution filters using MetaMorph software
Data qualification: Processed

• Cell Image Library Group ID: 9565

• Homo sapiens

• Cell
• Epidermal cell
• Keratinocyte

• Cell migration
• Substrate-dependent cell migration, cell attachment to substrate

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 13160

Doi: https://doi.org/10.6075/J0RN36K2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13160

Reference

• JCB 2009, 184:481-490 https://www.ncbi.nlm.nih.gov/pubmed/?term=19221195

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2022-08-12