Fischer, Robert S.; Waterman, Clare M. (2021). CIL:7428, Mus musculus, blood vessel endothelial cell, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0542MK6

Primary endothelial cells from transgenic mouse embedded in 3D collagen gels of ~ 100 Pa Young's modulus are exposed to 10 micromolar blebbistatin (myosin II ATPase inhibitor).
Phase contrast time lapse movie taken on a Nikon TE300 with a 20x 0.5 NA LWD objective. Images captured with an Orca II with 100 ms exposure. Image size is 0.1274 micron/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds
See Fischer et al for details. http://www.ncbi.nlm.nih.gov/pubmed/19185493

• 2021

• Fischer, Robert S.
• Waterman, Clare M.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro; 3D matrix
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Mus musculus

• Blood vessel endothelial cell
• Cell
• Extracellular matrix
• Primary cell line cell

• Cell migration
• Myosin II ATPase inhibition

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7428

Doi: https://doi.org/10.6075/J0542MK6

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7428

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=19185493

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2022-08-12