Allen, K.D.; Duysen, M.E.; Staehelin, L.A. (2021). CIL:39079, Triticum sp., leaf. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09885NM

CD3 mutant wheat (deficient in chlorophyll b was germinated and grown for 6 days in the dark, greened at high light intensity for 4 days in the presence of chloramphenicol, then prepared for thin section electron microscopy. The chloroplast shows a rather disorganized internal structure although grana stacks of thylakoids (GT) and unstacked stromal regions (ST) are evident. Also seen are osmiophilic globules (PG). V, vacuole, PM, plasma membrane, CW, cell wall. This set of grouped images, which also shows freeze-fracture views, includes wildtype chloroplasts.

• 2021

• Allen, K.D.
• Duysen, M.E.
• Staehelin, L.A.

Leaf segments 1 cm from the tip were fixed with 2% glutaraldehyde, postfixed with 1% osmium tetroxide, dehydrated, and embedded in Spurrs resin. Thin sections were stained with uranly acetate and lead citrate and examined in a JEOL 100C transmission EM at 60 KV, and images recorded on film. See Fig 12 in K D Allen et al. 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: film
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Processed

• Cell Image Library Group ID: 11355

• Triticum sp.

• Chloroplast
• Leaf
• Plastid thylakoid membrane

• Organelle organization
• Photosynthesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39079

Doi: https://doi.org/10.6075/J09885NM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39079

Reference

• Allen KD, Duysen ME, Staehelin LA. Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol. 1988 Sep;107(3):907-19. https://doi.org/10.1083/jcb.107.3.907
• K D Allen, ME Duysen, and LA Stahelin 1988 Biogenesis of thylakoid membranes is controlled by light intensity in the conditional chlorophyll b-deficient CD3 mutant of wheat. J Cell Biol 107:907-919 https://www.ncbi.nlm.nih.gov/pubmed/?term=3047153

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12