Apaja, Pirjo M.; Xu, Haijin; Lukacs, Gergely L. (2021). CIL:13683, Chlorocebus aethiops. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XK8D9Q

To study the cellular consequences of misfolding of plasma membrane proteins, a transmembrane model protein that was constitutively targeted to the plasma membrane was developed for use as a reporter molecule. The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). The CD4tl-lambdaC cytosolic domain was largely in native state at 26°C but predominantly nonnative state at 37°C thus allowing the use of thermal shifts to follow the fate of unfolded proteins. In this image, one of six of a group from Figure 1 of Pirjo et al., JCB 2010, the expression of the mutant plasma membrane reporter, CD4tl-lambdaC, at 37 degrees is seen with CD4 antibody (green) in non-permeabilized COS7 cells. The intracellular processing of this nonnative, thermally unfolded protein nearly completely limits its targeting to the plasma membrane. The ER marker, calreticulin antibody (red), is not apparent here because lack of detergent-permeabilization limited access of the antibody to the ER.

• 2021

• Apaja, Pirjo M.
• Lukacs, Gergely L.
• Xu, Haijin

Preparation: crosslinking-fixative fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: Alexa Fluor 488; Alexa Fluor 555
Processing history: unprocessed raw data
Data qualification: Raw

• Cell Image Library Group ID: 7831

• Chlorocebus aethiops

• Endoplasmic reticulum
• Integral to plasma membrane

• COS-7
• Protein targeting to plasma membrane

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13683

Doi: https://doi.org/10.6075/J0XK8D9Q

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13683

Reference

• JCB 2010, 191:553-570 https://www.ncbi.nlm.nih.gov/pubmed/?term=20974815

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2022-08-12