Buchanan, JoAnn (2021). CIL:6519, Danio rerio, Outer plexiform layer of retina. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GQ6WSC

This study of the zebrafish eye is a series of four images taken at increasing magnifications. The first image is the SEM block face of the zebrafish eye. This is followed by a second image that is a higher magnification TEM image of the plexiform layer and the outer nuclear layer of the zebrafish retina. The third image is a 15,000X TEM image of the bipolar and horizontal cells interdigitating in the OPL layer of the zebrafish retina. This image, the fourth in the series, is a higher magnification (60,000X) of the dense synaptic bar and synaptic vesicles.

Synaptic vesicles (V) surround the dense bar (DB) in the synaptic ribbon complex in the outer plexiform layer of the larval zebrafish retina. 10 dpf Image collected on a JOEL 1230 at 80 kV using a Gatan 967 slow-scan, cooled CCD camera at x60,000. 70 nm thick section.

Microwave processing, osmium tetroxoxide/osmid procedure for enhancing membranes, and copper lead en bloc staining.
Procedure for specimen preparation available at http://www.stanford.edu/~redhair/JoAnn_Buchanan.

• 2021

• Buchanan, JoAnn

Preparation: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue; heat fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; lead salt; copper salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1769

• Danio rerio

• Outer plexiform layer of retina
• Presynaptic active zone membrane
• Retina
• Ribbon synapse
• Synapse

• Synapse organization
• Synaptic transmission

• data
• image

• No linguistic content; Not applicable

Samplenumber: 6519

Doi: https://doi.org/10.6075/J0GQ6WSC

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL6519

Other resource

• JoAnn Buchanan: https://alleninstitute.org/what-we-do/brain-science/about/team/staff-profiles/joann-buchanan/
• Related resource: https://www.stanford.edu/~redhair/JoAnn_Buchanan/Zebrafish_Retina.html

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12