Sinclair, Paul; Bian, Qian; Plutz, Matt; Heard, Edith; Belmont, Andrew (2021). CIL:30552, Mus musculus, embryonic stem cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RX99Q4

z-series image of 3D immuno-FISH of multi-copy alpha-globin BAC insertions in a mouse ES cell. Immunostaining of HP1alpha (blue) is shown versus FISH signals against total BAC sequence (red) or combined lac operator and vector backbone sequences (green).

• 2021

• Belmont, Andrew
• Bian, Qian
• Heard, Edith
• Plutz, Matt
• Sinclair, Paul

Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. Pixel dimensions are 0.067 microns (xy) and z-spacing is 0.2 microns.
See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein; differences in adsorption or binding of stain
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); EGFP
Processing history: constrained iterative deconvolution
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 10842

• Mus musculus

• Embryonic stem cell
• Heterochromatin
• Nuclear chromatin
• Transgene

• Chromatin organization
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 30552

Doi: https://doi.org/10.6075/J0RX99Q4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL30552

Reference

• P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776. https://www.ncbi.nlm.nih.gov/pubmed/?term=20819934

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2022-08-12