Allen, Richard (2021). CIL:12340, Euplotes sp., cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09K49C8

A view of the cytopharynx and its nascent food vacuole forming region. The flattened pharyngeal vesicles that may contain monolayers or bilayers of lipids seem to be equivalent to the discoidal vesicles of Paramecium that provide membrane for food vacuole growth. They approach and fuse with the cytopharyngeal membrane between the lamellae (cytopharyngeal ribbons). TEM taken on 7/29/67 by R. Allen with Philips 200 operating at 60kV. Neg. 9,000X. Bar = 0.5µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol, and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. There is a high resolution version of this image in the library (CIL:39114) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 9890

• Euplotes sp.

• Cell by organism
• Ciliated Protist
• Cortical cytoskeleton
• Eukaryotic cell
• Eukaryotic Protist
• Oral apparatus

• Cortical cytoskeleton organization
• Cytoplasm organization
• Euplotes sp. from Carolina Biological Supply
• Oral apparatus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12340

Doi: https://doi.org/10.6075/J09K49C8

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12340

Other resource

• Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/

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2025-01-30