Woodcock, Christopher; Miller, Gayle (2021). CIL:561, Acetabularia mediterranea, gamete. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X929H4

At cyst formation in the 'giant' unicellular green alga Acetabularia, each cyst contains a single nucleus which subsequently undergoes several rounds of mitosis prior to gamete formation. Initially the nuclei form a syncytium which separates into individual gametes. This section shows a stage in gametogenesis where the syncytium has just begun to divide into uninuclear gametes. See Woodcock and Miller, 1973. Ultrastructural features of the life cycle of Acetabularia I Gametogenesis. Proptoplasma 77, 313-319.

• 2021

• Miller, Gayle
• Woodcock, Christopher

Cysts were fixed in glutaraldehyde, post-fixed in osmium tetroxide, epoxy embedded, and thin sections stained with aqueous uranyl acetate and lead citrate. Grids were examined with a Philips 400 TEM operated at 80KV and images recorded on film.

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); illumination by electrons
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; osmium tetroxide; lead salt
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10296

• Acetabularia mediterranea

• Chloroplast
• Chloroplast starch grain
• Gamete
• Nucleus
• Plastid thylakoid membrane

• Gamete generation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 561

Doi: https://doi.org/10.6075/J0X929H4

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL561

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2022-08-12