Ivanchenko, Maryna V.; Cicconet, Marcelo; Jandal, Hoor Al; Wu, Xudong; Corey, David P.; Indzhykulian, Artur A. (2022). CIL: 50706, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 4.3_Cell_4_Raw_Image_Stack. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0765DQZ

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

• 2022

• Cicconet, Marcelo
• Corey, David P.
• Indzhykulian, Artur A.
• Ivanchenko , Maryna V.
• Jandal, Hoor Al
• Wu, Xudong

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
4.3_Cell_4_Raw_Image_Stack is a multipage TIFF file consisting of 368 individual EM sections representing the entire volume of Cell#4 (concatenated stack, 1.95×1.95×15 nm³ voxel size).
This file is a part of Dataset 4, comprised of files related to the Cell #4. The numbering order of the files reflects the order of operations performed during the analysis. Note: The data acquisition session was interrupted after frame #224, and later resumed as a separate imaging session. This resulted in two stack files, concatenated into a single multipage TIFF file during data analysis.

Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)
Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads

• Cell Image Library Group ID: 20004

• Mus musculus

• Cuticular plate
• Hair cell stereocilia
• Inner ear outer hair cell
• Mitochondria
• Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)

• Native auditory Hair Cells at postnatal day 4

• data
• image

• No linguistic content; Not applicable

Samplenumber: 50706

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL50706

Reference

• Nature Communications article: https://www.nature.com/articles/s41467-019-11712-w
• Scientific Data article: https://www.nature.com/articles/s41597-020-0509-4

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2022-11-18