Ivanchenko, Maryna V.; Cicconet, Marcelo; Jandal, Hoor Al; Wu, Xudong; Corey, David P.; Indzhykulian, Artur A. (2022). CIL: 50684, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles: 1.5_Cell_1_Stereocilia_Segmentation. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0XW4J6M

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

• 2022

• Cicconet, Marcelo
• Corey, David P.
• Indzhykulian, Artur A.
• Ivanchenko , Maryna V.
• Jandal, Hoor Al
• Wu, Xudong

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.
1.5_Cell_1_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results (2.43×2.43×15 nm³ voxel size). Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for its online preview. The file contains 348 images, as all the planes that contained no segmentation data were removed.
This file is a part of Dataset 1, comprised of files related to the Cell #1 of current study. The numbering order of the files reflects the order of operations performed during the analysis.

Imaging mode: Serial Electron Microscopy; Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)
Visualization methods: Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid); Immunogold labeling with 10 nm gold beads

• Cell Image Library Group ID: 20000

• Mus musculus

• Cuticular plate
• Hair cell stereocilia
• Inner ear outer hair cell
• Mitochondria
• Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)

• Native auditory Hair Cells at postnatal day 4

• data
• image

• No linguistic content; Not applicable

Samplenumber: 50684

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL50684

Reference

• Nature Communications article: https://www.nature.com/articles/s41467-019-11712-w
• Scientific Data article: https://www.nature.com/articles/s41597-020-0509-4

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2022-11-18