Allen, Richard (2021). CIL:36717, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01V5CWG

In some cells exposed to horseradish peroxidase (HRP) for 30 sec and then immediately fixed some HRP has already entered the cell by endocytosis and has been removed from the early endosomes in carrier vesicles. In this picture such carrier vesicles have already docked at acidosomes that are themselves docked at a nascent digestive vacuole. Carrier vesicles will fuse with the acidosomes to add their content and membrane to the acidosomes. This is why two populations of acidosomes are often seen around the nascent phagosome and early DV-I, acidosomes that contain HRP because they have fused with HRP-containing carrier vesicles and those that have not fused with HRP-containing carrier vesicles. (See Allen, Ma and Fok, J. Cell Sci. 106:411-422, 1993). TEM taken on 2/24/82 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 21,250X. Bar = 0.2µm.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed

• Paramecium multimicronucleatum

• Cell by organism
• Ciliated Protist
• Early endosome
• Endocytic vesicle lumen
• Eukaryotic cell
• Eukaryotic Protist
• Vesicle membrane

• Digestive system process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36717

Doi: https://doi.org/10.6075/J01V5CWG

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36717

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12