Woodcock, Christopher (2021). CIL:742, Necturus maculosus, nucleate erythrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05X285M

X-ray analysis shows that the nucleated erythrocytes of the amphibian Necturus maculosus (Mud Puppy) contains more ordered chromatin than most nuclei. Here, chromatin fibers were isolated from N. maculosus erythrocyte nuclei using mild micrococcal nuclease digestion, fixed briefly with glutaraldehyde, applied to thin carbon films and negatively stained with aurothioglucose. ~30 nm fibers are seen, some exhibiting short regions with apparent structural order.

• 2021

• Woodcock, Christopher

Preparation: unembedded tissue; glutaraldehyde fixed tissue
Relation to intact cell: isolated subcellular component
Item type: transmission electron microscopy (TEM)
Imaging mode: illumination by electrons; detection of electrons
Parameter imaged: electron density
Source of contrast: elastic scattering
Visualization methods: other negative stain
Processing history: raw unprocessed data

• Necturus maculosus

• Nuclear chromatin
• Nucleate erythrocyte

• Chromatin assembly
• DNA packaging

• data
• image

• No linguistic content; Not applicable

Samplenumber: 742

Doi: https://doi.org/10.6075/J05X285M

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL742

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=1757505
• Woodcock CL, Woodcock H, Horowitz RA. Ultrastructure of chromatin. I. Negative staining of isolated fibers. J Cell Sci. 1991 May;99 ( Pt 1):99-106. https://doi.org/10.1242/jcs.99.1.99

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2022-08-12