Price, Diana; Laakso, Aki; Cyr, Michele; Martone, Maryann; Yamada, Naoko; Thor, Andrea; Berlanga, Monica (2021). CIL:48013, Mus musculus, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P84C1X

Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite. This medium spiny neuron was contained in a thick section from the neostriatum of a dopamine transporter knock out mouse. The reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

• 2021

• Berlanga, Monica
• Cyr, Michele
• Laakso, Aki
• Martone, Maryann
• Price, Diana
• Thor, Andrea
• Yamada, Naoko

Micer were C57BL/129SvJ. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi 3MeV UHVEM. Single tilt images spanned -70 to 70° in 2° increments.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; tissue in epoxy resin embedment; osmium tetroxide fixed tissue
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: detection of electrons; illumination by electrons
Parameter imaged: electron density
Visualization methods: intracellular filling
Data qualification: Processed

• Cell Image Library Group ID: 17309

• Mus musculus

• CNS neuron (sensu Vertebrata)
• Dendrite
• Dendritic spine

• data
• image

• No linguistic content; Not applicable

Samplenumber: 48013

Doi: https://doi.org/10.6075/J0P84C1X

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL48013

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0Z037ZQ

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2022-08-12