Ball, David; Peccoud, Jean (2021). CIL:39661, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QR4X7C

10-hour timelapse of Wild-type Saccharomyces cerevisiae growing in 2% raffinose. These experiments are the control experiments used for comparison to the effects of the double mutant Clb2dbΔ, Clb5Δ on the cell cycle control system in budding yeast. The images from two experiments done with Wild-type Saccharomyces cerevisiae are available as CIL:39606-39625 and CIL:39646-39665. The images from two experiments done with the double mutant Clb2dbΔ, Clb5Δ are available as CIL:39626-39645 and CIL:39666-39685.

• 2021

• Ball, David
• Peccoud, Jean

Images were collected at 4-minute intervals on a DeltaVision system equipped with a with a 60x PlanApo
PH3 phase-contrast, oil immersion objective (NA = 1.4). This system has a motorized x-y-z stage, automatic focusing, motorized illumination shutters and a CoolSNAP_HQ2 camera.

Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10593

• Saccharomyces cerevisiae

• Cell surface

• Cell budding
• Cell cycle checkpoint
• Regulation of cell budding

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39661

Doi: https://doi.org/10.6075/J0QR4X7C

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39661

Reference

• Ball DA, Ahn TH, Wang P, Chen KC, Cao Y, Tyson JJ, Peccoud J, Baumann WT. Stochastic exit from mitosis in budding yeast: model predictions and experimental observations. Cell Cycle. 2011 Mar 15;10(6):999-1009. https://doi.org/10.4161/cc.10.6.14966

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2022-08-12