Microscopy product ID: 3629
Segmentation
Description | Manual delineation of the presynaptic calyx and post synaptic spine mat using Xvoxtrace 2.17. Also delineated were the locations of PSD's, vesicles, intracellular components of the post synaptic compartment. |
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Description | Zip file (~38 Mb) containing the Xvoxtrace files (*.trace) containing the manual tracings of the individual components of the calycyl synapse, including ER, PSD, vesicles, base, and spines. Each of these components was traced in a separate file and are contained in different subdirectories. Also included in each subdirectory are the surfaced contours in Synu (*.synu) format, along with the Viewdata file required for visualization with Synuview. |
Reconstruction
Description | Computed slice through a tomographic reconstruction of the pre- and post-synaptic structures of the calycal synapse onto a chick ciliary ganglion neuron. This slice was derived from the 3rd of 6 serial volumes comprising the entire reconstruction. |
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Description | Zip file containing entire serial tomographic volume in Analyze 7.5 format (embryo.hdr/embryo.img). This volume was creating by aligning and concatenating 6 serial tomograms. The volume file is 269.5 Mb. |
Animation
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Description | Downsampled animation (256x256) through the computed slices of the aligned and merged volumes showing the pre- and post-synaptic structures of the calycal synapse onto a chick ciliary ganlgion neuron. |
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- Cite This Work
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Shoop, Rick; Berg, Darwin; Ellisman, Mark H.; Yamada, Naoko; Esquenazi, Ed (2017). Microscopy product ID: 3629. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0FN160W
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
Strain: White leghorn
- Funding
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NIH
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Shoop RD, Esquenazi E, Yamada N, Ellisman MH, Berg DK (2002). Ultrastructure of a somatic spine mat for nicotinic signaling in neurons. J Neurosci, 22(3):748-756. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/11826104; Journal of Neuroscience. https://www.jneurosci.org/content/22/3/748
- Microscopy product 3629 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3629
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22