Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:34927, Xenopus laevis, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GH9GKS

Localization of cross-linking proteins in fibroblast cytoskeleton. Immuno-EM of the cell edge or interior (CIL 34929) of cytochalasin D-treated Xenopus fibroblasts stained with α-actinin primary antibody and 18-nm gold-conjugated secondary antibody. Gold particles (yellow) reveal α-actinin at filament crossovers in the cell interior. A fluorescence image of fibroblast lamellipodia double stained with TRITC-phalloidin (red) and either α-actinin (green) is available at CIL 34924. The image corresponds to Figure 5f from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: permeabilized tissue; glutaraldehyde fixed tissue; methanol fixed tissue; phalloidin
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; primary antibody plus labeled secondary antibody; platinum replica; immuno-gold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Xenopus laevis

• Actin cytoskeleton
• Alpha actinin
• Cell edge
• Fibroblast

• Branching of actin filaments
• Cytochalasin D treatment

• data
• image

• No linguistic content; Not applicable

Samplenumber: 34927

Doi: https://doi.org/10.6075/J0GH9GKS

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL34927

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12