Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23045, Rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04T6H7K

Preferential distribution of plectin in peripheral regions. REF-52 cells were treated with nocodazole to depolymerize MTs and induce collapse of most IFs to the cell center. Electron microscopy of peripheral cell regions after gelsolin treatment and immunogold (10 nm) labeling for plectin. Short intermediate filaments at the periphery have numerous plectin sidearms.
Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.
• Verkhovsky, Alexander B.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; tannic acid
Relation to intact cell: platinum replica
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: uranyl salt; shadowing and plating; primary antibody plus labeled secondary antibody; immunogold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8924

• Rattus

• Fibroblast
• Intermediate filament
• Plectin

• Gelsolin treatment
• Nocodazole treatment
• REF-52

• data
• image

• No linguistic content; Not applicable

Samplenumber: 23045

Doi: https://doi.org/10.6075/J04T6H7K

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL23045

Reference

• J Cell Biol. 1996 Nov;135(4):991-1007 https://www.ncbi.nlm.nih.gov/pubmed/?term=8922382

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2022-08-12