Ris, Hans (2021). CIL:41710, Cricetulus griseus, ovary. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JD4W52

High Voltage (1MeV) EM image of a metaphase chromosome isolated from CHO cells using a non-aqueous technique, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 55 degrees. The accompanying image has a tilt of 45 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.

• 1977-07-24

• 2021

• Ris, Hans

Cells were metaphase arrested with colcemid, then exposed to a low ionic strength medium (0.07M phosphate buffer)containing 50% D20 as a microtubule stabilizer. Preparations were squashed in 3:1 acetic acid:ethanol,stained, and transferred to an EM grid.
See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Preparation: chemically fixed tissue
Relation to intact cell: squash
Item type: film
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 12260

• Cricetulus griseus

• Nuclear chromosome
• Ovary

• CHO
• Chromosome organization
• Mitosis
• Mitotic metaphase

• data
• image

• No linguistic content; Not applicable

Samplenumber: 41710

Doi: https://doi.org/10.6075/J0JD4W52

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL41710

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2023-08-17