CIL:12101, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:12101, Colpoda cucullus, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J102CH
- Description
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Colpoda is a soil ciliate and this micrograph is characterized by numerous mucocysts and food vacuoles containing bacteria in various stages of digestion. This electron micrograph is near the posterior end of the cell and contains spongiome tubules of the contractile vacuole. Rows of cilia cover the cell and basal body/cilium complexes are present as dikinetids, two complexes in each cortical unit. TEM taken on 5/31/69 by R. Allen with Philips 300 operating at 60kV. Neg. 5,000X. Bar = 1µm. The negative was printed to paper and the image was scanned with a flatbed scanner and the 1µm bar added in Photoshop. This digitized image is available for qualitative analysis. A high resolution image is available in this library (CIL:38831) and can be used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections were prepared at approximately 75nm thickness. Additional information is available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned to Photoshop.
Data qualification: Processed;spatialmeasurements - Series
- Scientific Name
- Anatomy
- Topics
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 12101
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12101
- Paramecium and Other Ciliates: Richard Allen's Image Collection. Pacific Biosciences Research Center. University of Hawaii at Manoa: https://www5.pbrc.hawaii.edu/allen/
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- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12