Ferenz, Nick; Wadsworth, Patricia (2021). CIL:40267, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PZ57KQ

Time lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at prophase in a vertical strip perpendicular to the spindle orientation. Microtubules in the activated strip show a variety of motions both toward and away from spindle poles. This contrasts with the exclusively poleward motion of spindle microtubules seen at metaphase (and illustrated in the grouped movie CIL:40266). The time series of images used to prpeare this movie is included in the group of images.
See Fig 1A in: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002

• 2021

• Ferenz, Nick
• Wadsworth, Patricia

Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp.
See also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: spinning disk confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Data qualification: Processed

• Cell Image Library Group ID: 10943

• Sus scrofa domestica

• Kidney epithelial cell
• Microtubule cytoskeleton

• LLC-PK1
• Microtubule cytoskeleton organization
• Mitosis
• Mitotic prophase

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 40267

Doi: https://doi.org/10.6075/J0PZ57KQ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40267

Reference

• NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002 https://www.ncbi.nlm.nih.gov/pubmed/?term=17671163

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2022-08-12