Allen, Richard (2021). CIL:39193, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06T0KXQ

Opercularia coarctata. Transmission electron micrograph of contracted cell body. This ciliate does not have rows of basal body/cilia over its whole surface but has cilia in the oral region and in a trochal band area. The pellicle is sculpted into ridges and grooves that circle the body. A layer of endoplasmic reticulum lies under the pellicle next to a layer of myonemal fibers. The macronucleus, contractile vacuole, mitochondria, and peristomeare evident in this view. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 4,270X.

• 2021

• Allen, Richard

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 10120

• Opercularia
• Opercularia coarctata

• Cell by organism
• Cell cortex
• Ciliated Protist
• Cytoplasm
• Eukaryotic cell
• Eukaryotic Protist
• Macronucleus
• Mitochondrion
• Oral apparatus

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Cortical cytoskeleton organization
• Cytoplasm organization
• Macronucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39193

Doi: https://doi.org/10.6075/J06T0KXQ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39193

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2022-08-12