Bushong, Eric A.; Martone, Maryann E.; Ellisman, Mark H. (2021). CIL:36312, Rattus, astrocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J03J3D1F

Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of 3 week old rat reveals highly ramified spongiform processes that span territories with minimal overlap. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled to produce this maximum intensity projection image.

• 2021

• Bushong, Eric A.
• Ellisman, Mark H.
• Martone, Maryann E.

Male Sprague Dawley rats, 3 weeks of age, were anaesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy,cells with a small diameter soma (approx. 7–11µm) in the stratum
radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using a 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.

Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed

• Cell Image Library Group ID: 9482

• Rattus

• Astrocyte
• Cell body
• Cell projection cytoplasm

• Forebrain astrocyte development

• data
• image

• No linguistic content; Not applicable

Samplenumber: 36312

Doi: https://doi.org/10.6075/J03J3D1F

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36312

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0FF3S5V

Reference

• Abiodun MO, Johnson TO. Liver function tests in Nigerian diabetics with particular reference to brom-sulphalein retention. West Afr Med J Niger Pract. 1972 Apr;21(2):47-50. PMID: 5036382. https://www.ncbi.nlm.nih.gov/pubmed/?term=5036382

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2022-08-12