Carpenter, Anne (2021). CIL:21748, Drosophila melanogaster, epithelial. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0JM28RJ

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi. This is the set that were treated with 340 RNAi. There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image. A tab-delimited text file containing this information can be found at the URL indicated in the attribution box. The recommended citation when using these images is: "We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."

• 2021

• Carpenter, Anne

Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of DNA
Visualization methods: Hoechst 33342
Processing history: unprocessed raw data

• Cell Image Library Group ID: 8115

• Drosophila melanogaster

• DNA
• Epithelial

• DsRNA 340 treatment
• Kc167

• data
• image

• No linguistic content; Not applicable

Samplenumber: 21748

Doi: https://doi.org/10.6075/J0JM28RJ

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL21748

Other resource

• Broad Bioimage Benchmark Collection (image "Drosophila Kc167 cells"): https://bbbc.broadinstitute.org/search/drosophila%20kc1671?

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2022-08-12