Kim, Sungsu; Wairkar, Yogesh P.; Daniels, Richard W.; DiAntonio, Aaron (2021). CIL:13468, Drosophila melanogaster, garland cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Q23XX2

Transmission electron micrograph of Drosophila nephrocyte garland cell from ema[1] mutant. ema mutant garland cells are filled with very large electron-lucent alpha and electron-dense alpha and beta vacuoles, many of which are 5–10 microns in diameter, whereas the largest vacuoles in wild type are ∼2 microns in diameter. In wild type, both types of vacuoles usually contain a single aggregate of electron-dense granular material that appears to be attached to the limiting membrane. However, the large vacuolar compartments in the mutant contain numerous electron-dense granular structures, suggesting they arise from homo-/heterotypic fusions of alpha and beta vacuoles. Samples for TEM were fixed for 1 h at 4°C in 2% paraformaldehyde, 2% glutaraldehyde, and 1% tannic acid in 0.1 M cacodylic acid buffer, pH 7.2. Then, the samples were postfixed in 1% OsO4 in 0.1 M cacodylic acid buffer, pH 7.2, for 1 h at room temperature, stained en bloc with 1% uranyl acetate, dehydrated in a grade series of ethanol and propylene oxide, and embedded in epon resin (Electron Microscopy Sciences). Blocks were sectioned in an ultramicrotome (RMC Products) at ∼70-nm thickness with a Delaware Diamond knife and post-stained for 1 h in Reynolds lead citrate and uranyl acetate. Electron micrographs were taken on a transmission electron microscope (H-7500; Hitachi) using an AMT camera system. Mag: 4000x. Image corresponds to Figure 2B, right ema[1] panel in Kim et al. J Cell Biol. 188: 717-734. 2010. High magnification image taken from this micrograph is in CIL# 13469.

• 2021

• Daniels, Richard W.
• DiAntonio, Aaron
• Kim, Sungsu
• Wairkar, Yogesh P.

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image; charge coupled device (CCD)
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: uranyl salt; lead salt; osmium tetroxide
Data qualification: Raw;intensitiesquantitation

• Cell Image Library Group ID: 6022

• Drosophila melanogaster

• Early endosome
• Garland cell
• Late endosome

• Ema[1]
• Endocytosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 13468

Doi: https://doi.org/10.6075/J0Q23XX2

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL13468

Reference

• J Cell Biol. 188: 717-734. 2010 https://www.ncbi.nlm.nih.gov/pubmed/?term=20194640

Other resource

• BioStudies (previously JCB DataViewer): https://www.ebi.ac.uk/biostudies/studies/

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12