Howard, Louisa (2021). CIL:39788, Lytechinus pictus, mesenchymal cell, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TM79F9

Scanning electron microscope image of Strongylocentrotus drobachiensus embryo at the primary mesenchyme blastula stage. Embryo was split open to reveal the outer epithelial layer and the blastocoel cavity at the vegetal pole. At this stage of embryonic development, primary mesenchyme cells are forming (flask shaped cells in the epithelial layer) and beginning to migrate into the blastocoel cavity.This image is part of a group of SEM images of sea urchin embryos at various stages of development. The group is available as CIL:39782-39791.

• 2021

• Howard, Louisa

Images taken on a JEOL 35C SEM.
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.

Preparation: glutaraldehyde fixed tissue; critical_point dried specimen; ethanol dehydration
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: scanning electron microscopy (SEM)
Parameter imaged: elastic scattering of electrons
Source of contrast: differences in deposition of metal shadow
Visualization methods: gold; visualization of contiguous regions; osmium tetroxide
Processing history: unprocessed raw data

• Cell Image Library Group ID: 10724

• Lytechinus pictus

• Cell surface
• Epithelial cell
• Mesenchymal cell

• Cell migration
• Embryonic morphogenesis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39788

Doi: https://doi.org/10.6075/J0TM79F9

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39788

Other resource

• Dartmouth College Electron Microscope Facility (search on the string "sea urchin"): https://www.dartmouth.edu/emlab/gallery/index.php

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12