Xu, Z.; Ogawa, H.; Vagnarelli, P.; Bergmann, J.H.; Hudson, D.F.; Ruchard, S.; Fukagawa, T.; Earnshaw, W.C.; Samejima, K. (2021). CIL:12228, Gallus gallus gallus, memory B cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0W957VM

Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Anaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP WT and treated with ZM44749. Endogenous Aurora B is green and DAPI (DNA) is blue. See Supplementary Fig 5A panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.

• 2021

• Bergmann, J.H.
• Earnshaw, W.C.
• Fukagawa, T.
• Hudson, D.F.
• Ogawa, H.
• Ruchard, S.
• Samejima, K.
• Vagnarelli, P.
• Xu, Z.

Preparation: formaldehyde fixed tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: widefield illumination
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: Fluorescein (FITC); TexasRed; 4',6-diamidino-2-phenylindole (DAPI)
Processing history: constrained iterative deconvolution
Data qualification: Processed

• Cell Image Library Group ID: 4151

• Gallus gallus gallus

• Chromosome, centromeric region
• Memory B cell
• Nuclear chromosome
• Spindle

• DT40
• Mitosis

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12228

Doi: https://doi.org/10.6075/J0W957VM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12228

Reference

• JCB 178:1207-1221, 2007 https://www.ncbi.nlm.nih.gov/pubmed/?term=17893245
• Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J Cell Biol. 2007 Sep 24;178(7):1207-21. https://doi.org/10.1083/jcb.200706012

Other resource

• BioStudies (previously JCB DataViewer) [search for content released in 2014]: https://www.ebi.ac.uk/biostudies/JCB/studies?#

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License

• UC Regents

Under copyright (US)

Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.

Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12