LaFountain, James R.; Oldenbourg, Rudolf (2021). CIL:12026, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J089150G

Movie of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30/60/90 sec for 2 hr 31 min using a QImaging Retigo EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

• 2021

• LaFountain, James R.
• Oldenbourg, Rudolf

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm

• Cell Image Library Group ID: 11200

• Nephrotoma
• Nephrotoma suturalis

• Nuclear chromosome
• Primary spermatocyte
• Spindle

• Male meiosis chromosome segregation

• data
• image

• No linguistic content; Not applicable

Samplenumber: 12026

Doi: https://doi.org/10.6075/J089150G

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL12026

Reference

• LaFountain JR, Oldenbourg R (2004) Mol Biol Cell 15, 5346-5355. https://doi.org/10.1091/mbc.e04-06-0524
• Oldenbourg R, Mei G (1995) J Microsc 180, 140-147.
• PubMed article: <@ https://www.ncbi.nlm.nih.gov/pubmed/?term=8537959
• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=12790452
• Shribak M, Oldenbourg R (2003) Appl Opt 42, 3009-3017 https://www.ncbi.nlm.nih.gov/pubmed/?term=15385630
• Shribak M, Oldenbourg R. Techniques for fast and sensitive measurements of two-dimensional birefringence distributions. Appl Opt. 2003 Jun 1;42(16):3009-17. https:/doi.org/10.1364/ao.42.003009

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2022-08-12