CIL:9064, Nephrotoma suturalis, spermatocyte
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- Cite This Work
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Oldenbourg, Rudolf; LaFountain, James R. (2021). CIL:9064, Nephrotoma suturalis, spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NC607J
- Description
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The events of meiosis I in a living insect spermatocyte beginning at metaphase with bivalents at the spindle equator, followed by anaphase onset, chromosome segregation and the start of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 60 sec over 105 min by a QImaging Retigo EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white).
- Date Issued
- 2021
- Researchers
- Technical Details
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Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image; charge coupled device (CCD); Processed image based on 5 recorded images
Imaging mode: polarization microscopy
Parameter imaged: UV/visible/IR photons
Source of contrast: intrinsic birefringence
Visualization methods: relative retardation (retardance) measurement
Processing history: 5-frame algorithm - Series
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- No linguistic content; Not applicable
- Identifier
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Samplenumber: 9064
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9064
- PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=15385630
- Shribak M, Oldenbourg R. Techniques for fast and sensitive measurements of two-dimensional birefringence distributions. Appl Opt. 2003 Jun 1;42(16):3009-17. https:/doi.org/10.1364/ao.42.003009
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12