Microscopy product ID: 9526
Reflection contrast microscopy overview of a glomerular section after chronic infusion of inactive hyaluronidase. Presence of DAB stained albumin results in a red reflection, which is found around glomerular capillaries, specifically at podocyte surface. The sample has been imaged at 46 nm pixel-1 resolution over an area of 109 x 131 ╡m2. See MP9525: D2_RK_Ab1to100_18500x_stitch for an adjacent section imaged with TEM.
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Mosaic of a a glomerular section after chronic infusion of inactive hyaluronidase (control) imaged with reflection contrast microscopy. The sample has been imaged at 46 nm pixel-1 resolution over an area of 109 x 131 ╡m2. See MP9525: D2_RK_Ab1to100_18500x_stitch for an adjacent section imaged with TEM.
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- Experiment: 2011-01-01. Project: 2009-09-01 to 2013-09-01.
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- Technical Details
Male. Strain: C57BL/6
Product type: MOSAIC. Microscopy type: TRANSMITTED LIGHT. Instrument: Leitz Orthoplan microscope equipped for epi-illumination, and adapted for reflection contrast microscopy
- Related Publications
Dane MJC, van den Berg BM, Avramut MC, Faas FGA, van der Vlag J, Rops ALWMM, Ravelli RBG, Koster BJ, van Zonneveld AJ, Vink H, Rabelink TJ (2013). Glomerular endothelial surface layer acts as a barrier against albumin filtration. Am J Pathol, 182(5):1532-1540. doi: 10.1016/j.ajpath.2013.01.049 PMID: 23518410
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Dutch Kidney Foundation
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