CIL:40217, Mus musculus, Neuroblastoma
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- Collection
- Cite This Work
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Yu, Weimiao; Lee, Hwee Kuan; Hariharan, Srivats; Bu, Wen Yu; Ahmed, Sohail (2021). CIL:40217, Mus musculus, Neuroblastoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J05B02KW
- Description
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Wide field fluorescent image of cultured neuroblastoma cells labeled with phalloidn FITC (green) and DAPI (blue) nuclear stain. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For further information, see Weimiao Yu, Hwee Kuan Lee, Srivats Hariharan, Wen Yu Bu and Sohail Ahmed. Evolving Generalized Voronoi Diagrams of Active Contours for Accurate Cellular Image Segmentation. Cytometry Part A 77A pg. 379~386, 2010.
- Date Issued
- 2021
- Researchers
- Methods
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NIE115 neuroblastoma cells were grown on 18 X 18 mm lamin coated glass coverslips maintained at 37 deg C in 5% CO2. Culture medium was Dulbecoo's Modified Eagle's medium supllemented with 4500 mg/ml glucose and 1% penicillin-streptomycin as an antibiotic. Cells were serum starved for 48 hrs and then fixed with 4% paraformaldehyde, then counterstained with DAPI and FITC-phalloidin. Images were acquired with a Zeiss epifluorescence microscope and a 20X objective.
- Technical Details
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Preparation: formaldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: fluorescence microscopy
Parameter imaged: fluorescence emission
Source of contrast: differences in adsorption or binding of stain
Visualization methods: 4',6-diamidino-2-phenylindole (DAPI); Fluorescein (FITC)
Data qualification: Processed - Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 40217
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40217
- Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J09P31GZ
Source data
Previous version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12