Allen, Richard (2021). CIL:9702, Coleps hirtus, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06W9980

Section through the cytopharynx of Coleps showing the corrugated lips containing lamellae surrounding the atrium of the buccal cavity. Rods composed of hexagonally packed microtubules that support the buccal cavity are seen in the cytoplasm. At the periphery an algal endosymbiont enclosed within a membrane is observed. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 3/24/69 by R. Allen with Philips 300 operating at 60kV. Neg. 14,800X. Bar = 0.5µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:2869) is available for quantitative analysis.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: unprocessed raw data; film; Printed and scanned for Photoshop
Data qualification: Processed;spatialmeasurements

• Cell Image Library Group ID: 3236

• Coleps hirtus

• Buccal cavity
• Ciliated Protist
• Endosymbiotic algae
• Eukaryotic Protist
• Microtubule associated complex

• Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
• Digestive system process
• Structural molecule activity
• Symbiosis, encompassing mutualism through parasitism

• data
• image

• No linguistic content; Not applicable

Samplenumber: 9702

Doi: https://doi.org/10.6075/J06W9980

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9702

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12