CIL:36220, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
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- Collection
- Cite This Work
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Allen, Richard (2021). CIL:36220, Tetrahymena pyriformis, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07D2T47
- Description
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Some accessory microtubules from the proximal ends of membranelle basal bodies extend upward and curve over the left wall of the buccal cavity as can be followed in this micrograph. Some fibrous components (the posterior connectives, see Smith and Buhse, Trans. Am. Microsc. Soc. 102:264-271, 1983) from the membranelles pass into the specialized cytoplasm where it forms an electron opaque mass. The bottom of the buccal cavity bordered by oral ribs opens through the cytosome into the nascent digestive vacuole. The cytopharynx is the site of lamellar microtubules (1 or 2 microtubules per lamella) which direct flattened vesicles to the growing vacuole‚Äôs fusion site. TEM taken on 8/16/67 by R. Allen with Philips 200 operating at 60kV. Neg. 12,400X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39717) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Date Issued
- 2021
- Researcher
- Methods
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Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
- Technical Details
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Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; Print from negative scanned for Photoshop.
Data qualification: Processed - Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 36220
- Related Resource
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL36220
Source data
- License
- Copyright
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Creative Commons Public Domain Dedication
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Constraint(s) on Use: This work may be used without prior permission.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2022-08-12