Microscopy product ID: 3406
2D Image
Description | Single tilt image at zero degrees tilt of a 0.5 thick section of blue green algae grown under low light conditions showing the chloroplast and thylakoid membranes taken with intermediate voltage electron microscopy. Contrast is reversed so that electron dense structures appear bright. |
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Description | Tar file containing digitized full unaligned resolution images (*.f), along with the fiducial mark file (*.fido), the origin file (*.fido,origin) and the file containing the angles used in the reconstruction. |
Segmentation
Description | Segmentation of chloroplast outer membrane (yellow) and thylakoid inner membranes (green) followed by surfacing using Amira |
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Description | Tar file containing segmented chloroplast membrane and thylakoids in Synu (.synu), Amira (.iv) and Analyze 7.5 (.img/.hdr) format. File also contains the Xvoxtrace .trace file. |
Reconstruction
Description | Computed slice through a tomographic volume of a chloroplast from phaeocystis antartica, a blue green algae, grown under low light conditions. |
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Description | Tar file containing two volumes in Analyze 7.5 format: Phaeo1.150.img/hdr and Phaeo1.512.img/hdr. Phaeo1.512 contains the entire cell, although the voxels appear to be saturated in some cases. Phaeo1.150 contains only a single chloroplast (see image map for subvolume) and doesn't contain saturated voxels. |
Animation
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Description | Animation sequence through the computed slices of a tomographic reconstruction of the chloroplast and thylakoid membranes of phaeocystis antartica, a blue green algae. |
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- Collection
- Cite This Work
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Moisan, Tiffany; Sosinsky, Gina; Buitenhuys, Casey; Ellisman, Mark H. (2017). Microscopy product ID: 3406. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0ZS2W9M
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
Strain: Karsten
- Funding
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National Aeronautics and Space Administration
- Series
- Scientific Name
- Anatomy
- Topic
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- Language
- No linguistic content; Not applicable
- Related Resources
- Moisan TA, Ellisman MH, Buitenhuys CW, Sosinsky GE (2006). Differences in chloroplast ultrastructure of Phaeocystis antarctica in low and high light. Marine Biology, 149(6):1281-1290. https://doi.org/10.1007/s00227-006-0321-5
- Microscopy product 3406 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3406
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22