Tani, Tomomi; Allen, Richard (2021). CIL:9688, Paramecium multimicronucleatum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Q81C7S

Paramecium multimicronucleatum - Video of the Contractile Vacuole Complex. Activity of the two parts of a dissected CV. Division and subsequent fusion of an in vitro CV extruded from a ruptured Paramecium cell. A microneedle is used to pinch the CV into two parts. Both CV parts will undergo rounding and relaxing but these cycles soon go out of phase with each other. When the microneedle is removed the two parts of the CV will again fuse and the single CV will continue to undergo rounding and relaxing. A DVD resolution version of this is available at CIL:10415.

• 2021

• Allen, Richard
• Tani, Tomomi

Preparation: living tissue
Relation to intact cell: isolated subcellular component
Item type: microscopy; modes of light microscopy
Imaging mode: differential interference contrast microscopy
Parameter imaged: refractive index
Source of contrast: boundaries between regions with different refractive index
Visualization methods: miscellaneous fill
Processing history: film; unprocessed raw data

• Cell Image Library Group ID: 2661

• Paramecium multimicronucleatum

• Ciliated Protist
• Contractile vacuole
• Eukaryotic cell
• Eukaryotic Protist

• Cellular water homeostasis

• data
• image
• video

• No linguistic content; Not applicable

Samplenumber: 9688

Doi: https://doi.org/10.6075/J0Q81C7S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL9688

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=10607534
• Tani T, Allen RD, Naitoh Y. Periodic tension development in the membrane of the in vitro contractile vacuole of Paramecium multimicronucleatum: modification by bisection, fusion and suction. J Exp Biol. 2000 Jan;203(Pt 2):239-51. https://doi.org/10.1242/jeb.203.2.239

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2022-08-12