CIL:37162, Rattus, CNS neuron (sensu Vertebrata)
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- Collection
- Cite This Work
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Hodges, Jennifer L.; Newell-Litwa, Karen; Asmussen, Hannelore; Vicente-Manzanares, Miguel; Horwitz, Alan Rick (2021). CIL:37162, Rattus, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TQ61MW
- Description
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This is Video S3,which corresponds to still images in bottom row of Fig. 2D. It shows that knocking down levels of Myosin II B (MIIB) increases spine dynamics. Spines from MIIB knockdown neurons extend and retract more frequently than spines in control neurons. Note also the increased length of spine extensions in MIIB knockdown neurons. Timelapse confocal imaging was performed on DIV14 hippocampal neurons co-expressing GFP and an shRNA vector against MIIB. Images were acquired every 1-minute using confocal microscopy. 3 frames/sec shown.
- Creation Date
- 2011
- Date Issued
- 2021
- Researchers
- Methods
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Confocal images were collected on an Olympus Fluoview 1000 microscope (IX81 base) equipped with a 60X/1.35 NA (oil)UPLSAPO 60X objective (Olympus). GFP was excited using the 488 nm laser line of a multi Ar laser. Fluorescence emission was collected using the following dichroic mirror/filter: SDM560/BA505–525 (GFP).
- Technical Details
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Preparation: living tissue
Relation to intact cell: dispersed cells in vitro
Item type: time lapse microscopy
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: distribution of a specific protein
Visualization methods: EGFP
Processing history: unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation - Series
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- Language
- No linguistic content; Not applicable
- Identifier
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Samplenumber: 37162
- Related Resources
- Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL37162
- Hodges JL, Newell-Litwa K, Asmussen H, Vicente-Manzanares M, Horwitz AR (2011) Myosin IIB Activity and Phosphorylation Status Determines Dendritic Spine and Post-Synaptic Density Morphology. PLoS ONE 6(8): e24149. https://doi.org/10.1371/journal.pone.0024149
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- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-08-17