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CIL:31927, Homo sapiens, retinal pigment epithelium

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  • 1.86 GB
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Cite This Work

Hatano, Toshiyuki; Sluder, Greenfield (2021). CIL:31927, Homo sapiens, retinal pigment epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09022NB

Description

Time lapse movie of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. The time series of images used to prepare the movie is grouped with it in the Cell Image Library.

Date Issued
  • 2021
Researchers
Methods

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Technical Details

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: charge coupled device (CCD)
Imaging mode: phase contrast microscopy
Parameter imaged: elastic scattering of photons
Source of contrast: differences in amount of elastic light scattering
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

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  • No linguistic content; Not applicable
Identifier

Samplenumber: 31927

Doi: https://doi.org/10.6075/J09022NB

Related Resource
License

CC0 1.0 Universal Public Domain Dedication

Copyright

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Digital Object Made Available By

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

Last Modified

2022-08-12