Allen, Richard (2021). CIL:7336, Opercularia [NCBITaxon:168247], Opercularia coarctata, cell by organism, eukaryotic cell, Eukaryotic Protist, ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZC81W1

A high resolution image of the pellicle of Opercularia coarctata. Indentations of the plasma membrane penetrate the alveolar membranes end as coated pits. These pits are also known as pellicular pores are homologous to parasomal sacs of other ciliates. The surface ridges contain an electron opaque rod in the cytosol of unknown composition, seen here in cross section. TEM taken on 6/11/69 by R. Allen with Philips 300 operating at 60kV. Neg. 30,000X. The raw film was scanned with a Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

• 2021

• Allen, Richard

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation to intact cell: microtome-sectioned tissue
Item type: transmission electron microscopy (TEM); illumination by electrons
Imaging mode: detection of electrons; film
Parameter imaged: electron density
Source of contrast: stain with broad specificity
Visualization methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing history: recorded image; film; unprocessed raw data
Data qualification: Raw;spatialmeasurements;intensitiesquantitation

• Cell Image Library Group ID: 7236

• Opercularia
• Opercularia coarctata

• Alveolar membranes
• Cell by organism
• Ciliated Protist
• Clathrin coat of coated pit
• Eukaryotic cell
• Eukaryotic Protist
• Plasma membrane organization
• Rods of unknown material

• Cambridge Culture Collection, Cambridge, England, # LB1657/1
• Clathrin binding
• Clathrin coat assembly
• Cytoskeletal anchoring at plasma membrane
• Cytoskeletal organization
• Plasma membrane organization
• Plasma membrane to endosome transport

• data
• image

• No linguistic content; Not applicable

Samplenumber: 7336

Doi: https://doi.org/10.6075/J0ZC81W1

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL7336

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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12