Zaritsky, Assaf (2021). CIL:43415, Mus musculus, mammary adenocarcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PG1RWM

Wound Healing Assay
Time series DIC images of mouse DA3 cells, derived from the mouse mammary adenocarcinoma cell line D1-DMBA-3, induced in BALB/C mice by dimethylbenzanthracene were grown to 90% confluence. A scratch approximately 300um wide was made in the cell monolayer, and images were recorded every 14.5 min for 26 hr.
This time series image, obtained with no PHA and HGF/SF treatment, is part of a group of 17 time series images (CIL:43401 to CIL:43417) representing three different treatments: (1) no HGF/SF (CIL:43406 to CIL:43411), (2) with HGF/SF (CIL:43401 to CIL:43405), activating HGF/SF-Met signaling, and (3) with PHA (Met inhibitor) and HGF/SF (CIL:43412 to CIL:43417). The group also includes two movies (CIL:43418 and CIL:43419) created from the time series CIL:43402 and CIL:43406 respectively.

• 2021

• Zaritsky, Assaf

DA3 cells expressing the fluorescent protein mCherry were grown to 90% confluence in wells of 2 cm diameter in DMEM supplemented with 10% heat-inactivated FCS (Gibco-BRL) and treated with or without the Met inhibitor PHA665752 (2.5 µM) for 2 hrs. A scratch was generated using a 200 µl tip, and the cells were incubated with or without 80 ng ml-1 Met-Hepatocyte Growth Factor/Scatter Factor (HGF/SF) and subjected to time lapse confocal laser scanning microscopy (CLSM-510, Zeiss, Germany) for approximately 26 hrs, with images taken at 14.5 min intervals. The position of each scratch was predefined, and a macro that repetitively positions the microscope on each point was executed. The acquired differential interference contrast (DIC) channel of the time-lapse sequence (shown here) was used for the multi-cellular analysis; the red fluorescence channel was exploited for single cell tracking.
See also: Zaritsky A, Natan S, Ben-Jacob E, Tsarfaty I (2012). Emergence of HGF/SF -induced Coordinated Cellular Motility. PLOS ONE 7(9): e44671

Preparation: living tissue
Relation to intact cell: whole mounted tissue
Item type: recorded image
Imaging mode: differential interference contrast microscopy
Parameter imaged: optical path length gradient
Source of contrast: boundaries between regions with different refractive index
Visualization methods: visualization of contiguous regions
Processing history: unprocessed raw data

• Cell Image Library Group ID: 17052

• Mus musculus

• Mammary adenocarcinoma

• Cell migration
• DA3
• Wound healing

• data
• image

• No linguistic content; Not applicable

Samplenumber: 43415

Doi: https://doi.org/10.6075/J0PG1RWM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL43415

Reference

• Zaritsky A, Natan S, Ben-Jacob E, Tsarfaty I. Emergence of HGF/SF-induced coordinated cellular motility. PLoS One. 2012;7(9):e44671. https://doi.org/10.1371/journal.pone.0044671

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2022-08-12