Bushong, Eric A.; Martone, Maryann E.; Ellisman, Mark H. (2021). CIL:39858, Rattus norvegicus, astrocyte, astrocyte of the hippocampus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RN37ZD

Intracellular dye injections of protoplasmic astrocytes from the CA1 region of hippocampus of a 2 week old rat reveals highly ramified spongiform processes. Astrocytes were filled with Lucifer Yellow. Optical sections were generated with a single photon confocal microscope, and compiled here as a single maximum projection image. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

• 2021

• Bushong, Eric A.
• Ellisman, Mark H.
• Martone, Maryann E.

Male Sprague Dawley rats, 4 weeks of age, were anesthetized and perfused transcardially with with Ringer's solution (37°C), followed by 4% paraformaldehyde (PFA) in PBS (37°C, pH 7.4). Tissue was sectioned (100 µm thick) using a vibratome, and injected with a BX50WI microscope (Olympus, Melville, NY) and, using infrared differential interference contrast videomicroscopy, cells with a small diameter soma (approx. 7–11µm) in the stratum radiatum of CA1 were located and impaled with glass micropipettes filled with 5% aq. Lucifer Yellow. The astrocytes were filled using 0.5 Hz pulses of current until all of the processes were brightly fluorescent. After several astrocytes were filled, a slice was placed in the 4% PFA overnight at 4°C. Slices were then imaged after coverslipping in Gelvatol. Confocal images were acquired with a BioRad Radiance2000 (Hercules, CA) attached to a Nikon E600FN (Kanagawa, Japan) microscope equipped with a Nikon (Tokyo, Japan) 60x oil objective (NA 1.4). Additional details are provided in Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.

Preparation: formaldehyde fixed tissue
Relation to intact cell: vibratome-sectioned tissue
Item type: recorded image
Imaging mode: confocal microscopy
Parameter imaged: fluorescence emission
Source of contrast: intracellular filling
Visualization methods: lucifer yellow
Data qualification: Processed;spatialmeasurements

• Rattus norvegicus

• Astrocyte
• Astrocyte of the hippocampus
• Cell body
• Cell projection cytoplasm

• Cell projection organization
• Forebrain astrocyte development

• data
• image

• No linguistic content; Not applicable

Samplenumber: 39858

Doi: https://doi.org/10.6075/J0RN37ZD

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL39858

Previous version

• Item in Cell Centered Database, UC San Diego Library Digital Collections: https://doi.org/10.6075/J0CC10FV

Reference

• PubMed article: https://www.ncbi.nlm.nih.gov/pubmed/?term=5036382

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2022-08-12