Cronshaw, Janet; Matunis, Michael (2021). CIL:40318, Homo sapiens, epithelial cell, cervical carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZG6R1S

Transmission electron micrograph of a thin section of the nucleus of a HeLa cell expressing a mutant of the nuclear porin ALADIN. The nuclear envelope ultrastructure is not affected by the mutation. Other images in this group show details of nuclear pore structure in cross section and tangential section.

• 2021

• Cronshaw, Janet
• Matunis, Michael

Normal HeLa cells expressing were fixed with glutaraldehyde, embedded in epoxy resin, thin sectioned, and imaged using a Philips CM10 TEM.
See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827

Preparation: glutaraldehyde fixed tissue; tissue in plastic embedment
Relation to intact cell: microtome-sectioned tissue
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: electron density
Source of contrast: differences in adsorption or binding of stain
Visualization methods: osmium tetroxide; uranyl salt; lead salt
Data qualification: Processed

• Cell Image Library Group ID: 11064

• Homo sapiens

• Cervical carcinoma
• Epithelial cell
• Nuclear chromatin
• Nuclear envelope
• Nucleolus
• Nucleus

• HeLa
• Nuclear transport
• Nucleus organization

• data
• image

• No linguistic content; Not applicable

Samplenumber: 40318

Doi: https://doi.org/10.6075/J0ZG6R1S

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL40318

Reference

• J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827 https://www.ncbi.nlm.nih.gov/pubmed/?term=12730363

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2022-08-12