Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:24797, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X34WBM

Localization of Arp2/3 complex in the lamellioidia of afibroblast . Staining with p21 antibody and 10 nm gold secondary antibody. CIL 24794 shows a fluorescence microsopy image of Arp2/3 localization, also in fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3h from J Cell Biol. 1999 May 31;145(5):1009-26.

• 2021

• Borisy, Gary G.
• Svitkina, Tatyana M.

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Preparation: glutaraldehyde fixed tissue
Relation to intact cell: dispersed cells in vitro
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM)
Parameter imaged: optical path length gradient
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating; primary antibody plus labeled secondary antibody; immuno-gold
Processing history: unprocessed raw data

• Cell Image Library Group ID: 9875

• Actin cytoskeleton
• Fibroblast
• Lamellipodium

• Actin filament-based process

• data
• image

• No linguistic content; Not applicable

Samplenumber: 24797

Doi: https://doi.org/10.6075/J0X34WBM

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL24797

Reference

• J Cell Biol. 1999 May 31;145(5):1009-26. https://www.ncbi.nlm.nih.gov/pubmed/?term=10352018

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2022-08-12