Microscopy product ID: 3409
2D Image
Description | Zero tilt image of a 0.75 um thick section through a blue green algae imaged with intermediate voltage electron microscopy at 400 KeV. |
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Description | Tar file containing original full-size unaligned .f files in compressed format and the .crop files which have been cropped and aligned. |
Segmentation
Description | Manual tracing of the chloroplast structures, including the thylakoid membranes, the chloroplast membranes using Xvoxtrace 2.9 |
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Description | Tar file containing segmented objects in Synu format (*.synu) along with the Xvoxtrace file containing the manual tracings (phaeo4.trace) and the Viewdata file required to view the renderings in Synu. |
Reconstruction
Description | Single computed slice through a tomographic reconstruction of a blue green algae (phaeocystis antarctica) showing the thylakoid membranes and other internal structures. Dark speckles represent locations of colloidal gold that was placed on the surface to aid in tilt series alignment. |
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Description | Tar file containing the compressed volume file in Analyze 7.5 format. File also containing the fiducial mark file, the origin file, and the .ang file from Suprim and Xfido for alignment purposes. |
Animation
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Description | Aniimation through computed slices from a tomographic reconstruction of a blue green algae exposed to low light conditions. Dark speckles represent locations of colloidal gold that was placed on the surface to aid in tilt series alignment. |
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- Collection
- Cite This Work
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Moisan, Tiffany; Sosinsky, Gina; Buitenhuys, Casey; Ellisman, Mark H. (2017). Microscopy product ID: 3409. In Cell Centered Database. UC San Diego Library Digital Collections. https://doi.org/10.6075/J0KH0N4J
- Date Issued
- 2017
- Research Team Head
- Researchers
- Technical Details
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Product type: SINGLE TILT. Microscopy type: IVEM. Instrument: JEOL 4000EX IVEM
Strain: Karsten
- Funding
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National Aeronautics and Space Administration
- Series
- Scientific Name
- Anatomy
- Topic
Formats
View formats within this collection
- Language
- No linguistic content; Not applicable
- Related Resources
- Moisan TA, Ellisman MH, Buitenhuys CW, Sosinsky GE (2006). Differences in chloroplast ultrastructure of Phaeocystis antarctica in low and high light. Marine Biology, 149(6):1281-1290. https://doi.org/10.1007/s00227-006-0321-5
- Microscopy product 3409 at the Cell Centered Database: https://doi.org/10.7295/W9CCDB3409
Primary associated publication
Other version
- License
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Creative Commons Attribution 4.0 International Public License
- Rights Holder
- UC Regents
- Copyright
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Under copyright (US)
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
- Digital Object Made Available By
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Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
- Last Modified
2023-05-22