Ip, Wallace (2021). CIL:4656, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08S4P0N

Purified rabbit skeletal muscle myosin in 0.6M KCl was adsorbed onto mica flakes, quick frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress (Heuser JE, J. Mol.Biol. 1983. 169:155-195). The sample was freeze-fractured and deep-etched in a Balzers 400 freeze fracture device, and rotary-replicated in Pt-C at 11 degrees.

This image was recorded at 150,000x in a JEOL 100CX2 transmission electron microscope operating at 100 kev, on Kodak 4489 film. The image was digitized using a Nikon Coolscan 9000ED scanner at 20 micron pixel size.

• 2021

• Ip, Wallace

Preparation: unfixed tissue; freeze_fracture/freeze_etch; whole mounted tissue
Relation to intact cell: isolated subcellular component
Item type: recorded image
Imaging mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameter imaged: electron density; elevation
Source of contrast: differences in deposition of metal shadow
Visualization methods: shadowing and plating
Processing history: unprocessed raw data

• Cell Image Library Group ID: 1132

• Oryctolagus cuniculus

• Cytoskeleton
• Skeletal muscle cell

• ATPase activity, coupled to movement of substances
• Skeletal muscle contraction

• data
• image

• No linguistic content; Not applicable

Samplenumber: 4656

Doi: https://doi.org/10.6075/J08S4P0N

Source data

• Source Record in the Cell Image Library: https://doi.org/10.7295/W9CIL4656

CC0 1.0 Universal Public Domain Dedication

Creative Commons Public Domain Dedication

Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.

Constraint(s) on Use: This work may be used without prior permission.

Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)

2022-08-12